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KMID : 0829220050290020075
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Korean Journal of Oral and Maxillofacial Pathology
2005 Volume.29 No. 2 p.75 ~ p.81
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Anticancer effect of photodynamic therapy using 9HpbD-a and 632nm diode laser on human squamous cell carcinoma
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Oh Chung-Hun
Chun Gae-Sig
Jung Pil-Sang
Ahn Jin-Chul
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Abstract
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This study was conducted to test the anticancer effect of photodynamic therapy using chlorophyll derivative (9-HpbD-a) and 632nm diode laser. Human SNU 1041 cells were seeded into 96 well plate of 104cells/well and cultured for 24 hours. Cells were washed with media containing various concentration of 9-HpbD-a ranging from 0ug/ml to 3.75ug/ml. Then 632 nm diode laser was given at various lasering time setting, and at various starting time after initial 24 hours of culture. The treated cells were incubated 48 hours and tetrazolium-based colorimetric(MTT) assay was done to measure the viability of cells. For in vivo study, SNU-1041 cells were xenografted into the back of nude mouse. When the xenografted tumors grew up to 400-600 mm3, the animals were randomly placed into 4 groups: Group I (n=20), PDT group, interstitial injection of 9-HpbD-a (47 ug/kg) followed by irradiation with 3.2 J/㎠ of light 6 hours after then injection; Group II (n=10), irradiation with 3.2 J/㎠ of light using diode laser; Group III (n=10), interstitial injection of 9-HpbD-a only(47 ug/kg); Group IV (n=10), normal control group. The viability of cells was decreased with increasing lasering time. No significant difference of cell viability was noted by variously delayed starting time of lasering. PDT effects were observed in the xenografted nude mouse model. Group IV (no 9-HpbD-a, no laser irradiation) was a control group which showed a continuous tumor growth. Group III (9-HpbD-a injection only) showed no response, Group II (laser irradiation only) showed 1 complete remission out of 10 (10%), Group I (9-HpbD-a and laser irradiation) showed 13 cpmplete remission out of 20 (65%), Group I showed significant remission rate, comparing to other groups (p<0.05). This study demonstrated anticancer effect of photodynamic therapy using 9-HpbD-a and 632nm diode laser on human squamous cell carcinoma cell line.
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KEYWORD
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Photodynamic therapy, 9-hydroxypheophorbide-a, 632 nm diode laser
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